High-yield production of active recombinant S. simulans lysostaphin expressed in E. coli in a laboratory bioreactor

Staphylococcus aureus (S. aureus), which has developed multidrug resistance, leads to many healthcare-associated infections resulting in significant medical and economic losses. Therefore, the development of new efficient strategies deal with these bacteria been gaining importance. Lysostaphin is a peptidoglycan hydrolase that considerable potential as bacteriocin. However, there have few reported optimization scale-up studies lysostaphin bioproduction process. Our preliminary results revealed composition auto-induction media at 30 °C increases produced around 10-fold shake flasks. In this study, achieving higher yields for recombinant E. coli laboratory scale aim, through use media. Optimized medium fermentation parameters were transferred laboratory-scale bioreactor. The tested conditions improved protein up 184 mg/L 3 L stirred bioreactor productivity was 2-fold comparison previously published reports. Furthermore, study also showed an effective bacteriocin on both commercially available isolated S. strains. These will contribute future larger-scale production via proposed conditions.